Species:

Universal

Alternative Name:

RT3; r-T3; Reverse T3; (2S)-2-Amino-3-[4-(4-Hydroxy-3,5-Diiodophenoxy)-3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine

Assay Type:

Competitive

unit:

pg/mL

Standard:

6000 pg/mL

Sensitivity:

27.55 pg/mL

Detection Range:

93.75-6000 pg/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma and Other biological fluids

Research:

Infection immunity;Endocrinology;Hormone metabolism;Autoimmunity;

Test Principle:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with rT3. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to rT3. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.